| 1. | Resistance of the transgenic cho cell to toxicity
工程细胞株的建立及其应用 |
| 2. | Medical ethics in tissue engineering
骨组织工程细胞外基质材料 |
| 3. | These residues are mutated into alanine , either individually or in combinations of two to three residues
用gdnf分别刺激工程细胞， 3天后观察其存活和分化情况。 |
| 4. | Inhibitory effect of surface modification on chrysotile - induced changes of intracellular free calcium ion in human embryo lung cells
工程细胞株的建立及乙酰胆碱对其细胞内钙离子浓度的影响 |
| 5. | However , this approach ( generically termed " cell therapy " and including stem cell therapy ) still has limitations : the cells that the engineered cells are designed to replace must be eliminated
然而，这种方法（一般称为"细胞疗法" ，包括干细胞疗法）仍然有局限性：工程细胞被设计去置换的那些细胞必须被除去。 |
| 6. | Thus , strenghtening the basic biology theorical study of the engineering cell and seeking the better cell system for the baculovirus of silkworm are current needs for the study of silkworm expression system
因此，加强对工程细胞的生物学基础理论研究，寻找更适宜于家蚕杆状病毒表达系统的家蚕细胞系是当前家蚕表达系统研究发展的需要。 |
| 7. | It was applied widely to various fields of biology , medicine and agriculture . as engineering cells in the insect baculovirus expression system , insect cells have close relationship with to transform and expression efficiency in the expression system
昆虫细胞作为昆虫杆状病毒表达系统中的工程细胞，与表达系统的转化和表达效率密切相关，因此，昆虫细胞的研究对于表达系统的开发具有深远的影响作用。 |
| 8. | The mutants were transfected into pc 12 cells respectively to probed the ability respondence to gdnf stimulation and the interaction with ret by immunoprecipitation , c - ret phosphorylation and immunoblotting assays . the main results are as follows : 1 . expression and purification of recombinant rat gfral protein to obtain recombinant gfral and study its biological activity , the cdna encoding the mature rat gfral was isolated using rt - pcr with total rna extracted from newborn s
纯化和复性后的重组gdnf蛋白，可显著增强pc12一gfral一ret工程细胞的存活和分化;对pc12一ret工程细胞没有任何作用;对pc12一gfral的存活和分化作用11中英文摘要显著强于对pc12一ret的作用，但也显著低于对pc12一gfral一ret细胞的作用。 |
| 9. | This dissertaion , on the basis of the other studies , on the one hand , researched and analysed the chromosomes and mitoses of the cell line - bmn , which is widely used in the silkworm baculovirus expression system as an engineering cell . on the other hand , the dissertation attempted to explore establishment of the new silkworm embryoni cell line by primary culture
同时，家蚕体外细胞培养研究的进行，不仅可以为家蚕细胞生物学的基础理论研究提供良好的研究系统，而且在家蚕资源的开发与利用方面也具有重大的意义。本研究在借鉴前人研究的基础上，一方面对现在广泛应用于家蚕杆状病毒表达系统的工程细胞? bmn细胞的染色体和有丝分裂进行了研究与分析。 |
| 10. | The abilities of cultured cells mentioned above cannot be obtained just by optimization of medium formulations and the implementation of high - performance bioreactor configurations . modifying the cells themselves by gene technology to change their physiological exhibitions may provide a better way to make the cells more robust to challenging environments
工程细胞上述能力的获得仅由培养基配方和培养条件优化设计很难实现，必须从工程细胞本身着手，对细胞本身的生理特征进行改造，才有可能实现。 |
