| 1. | ; sabc and tunel kit were from boster co . , ltd . 3
Sabc和tunel染色试剂盒购于中国bos几r公司。 |
| 2. | Tea treatment also reduced the number of tunel positive cells and caspase - 3 activation
500pmtea还可减少tunel阳性细胞及caspase工激活。 |
| 3. | Fig . 1 the apoptotic cells in ca1 sector , chromatin condensed and stained blue , apoptotic bodies edhibited ( tunel method 1000 )
1 ca1区的凋亡细胞，染色质固缩蓝染并有凋亡小体形成（ tunel法1000 ） 。 |
| 4. | However , a - type channel blocker 4 - ap and channel blocker apamin displayed no effect on cell death and percentage of tunel positive cells and caspase - 3 activation
通道阻断剂叩amin门卜m ）对缺氧复氧后神经元死亡、 tunel阳性细胞比例以及caspascs激活均无显著影响，表明bkc 。 |
| 5. | By computer image analysis system nyd - 1000 analysing the results of tunel and immunohistochemistry , accounting the size and positive cell of egg granulomas , apoptotic and positive index of granuloma cell was determined
原位杂交实验结果显示，实验纽b ； ix和尬l 。 rna表达匀高于对买组。 |
| 6. | Cell injury was investigated by lactate dehydrogenase ( ldh ) activity in culture medium . and cell apoptosis was examined by flow cytometry , tunel staining and transmission electron microscopy ( tem )
重度低氧（ 2 3 oz ） 24h ，心肌细胞数和蛋白质合成均降低，并且培养液中ldh水平增高，提示重度低氧有细胞毒性作用。 |
| 7. | On this condition , we exert two different levels of fluid shear stress ( 4 dyns / cm2and 15 dyns / cm2 ) to them by a parallel plate flow chamber and observe the change of apoptosis of endothelial cells by tunel . we demonstrate that high level shear stress can inhibit the apoptosis induced by lps , whereas low level shear stress was less effective
在此基础上，将两种不同大小剪切力与lps共同作用于细胞，发现高水平剪切力( 15dyn cm ~ 2 )可以抑制lps诱导的凋亡，而低水平剪切力( 4dyn cm ~ 2 )的效果则不明显。 |
| 8. | From the view of cellular rheology , we examined the influence of fluid shear stress on the metabolism , morphology , structure and function of endothelia cells in our previous research . it is first time for us to develop the research between shear stress and apoptosis . in this study , we stimulate the human umbilical venous endothelial cells ( huvec ) by lps and apply different levels of fluid shear stress to them by a parallel plate flow chamber , then observe the change of apoptosis of endothelial cells by terminal deoxynucleotidyl transferase mediated dutp nick end labeling ( tunel )
我们从细胞力学的角度，曾对血管内皮细胞在剪切力作用下的代谢、形态、结构及功能等进行过系统研究，但剪切力与内皮细胞凋亡的相关研究尚属首次，我们希望通过探讨细胞凋亡与剪切力大小及作用时间的关系，为探索剪切力对血管内皮细胞信号转导的影响及作用机理提供有价值的参考数据和实验资料。 |
| 9. | Labeling tunel method . the cell ultrastructural changes were similar to apoptosis in animal cells : the apical meristemetic cells underwent the programmed cell death . this was first detected in the apex cells of apical meristem , while peripheral cells differentiated gradually into different parts of a floral bud . but all the cells in the floral bud were subjected to the pcd process before it developed into a complete flower . 140bp dna fragment was found to deposit in apical bud during the plant development . the most important role of caspase - 8 was detected by western blot , and the expression of the procaspase - 8 was time - related with the dna frgmentation and the transformation from vegetative to the reproductive growth . these results suggested that pcd was an active process during the differentiation of apical meristem , and the senescence observed in the apical bud was due to the pcd process
显微超微结构研究表明，短日照条件下豌豆顶芽的衰老过程是从营养生长锥向花芽的转化，而用dna原位末端标记tunel caspase - 8 western blot和140 bp dna片断积累的试验结果证明，转化为花芽的整个生长锥细胞发生了编程性死亡pcd ，而且其最顶端部分细胞首先发生pcd ，而顶端周围的分生组织细胞逐渐分化出花芽的各部分，但顶芽最后并没有发育成为完整的花，所有细胞就都发生pcd ，从而顶芽衰老。 |
| 10. | Then several kinds of selective k + channel blockers were applied to further clarify the specific type of k + channels involved in apoptosis . both bkca channel blockers , ibtx and chtx ( at the concentration of 100 nm ) , significantly reduced neuronal death and number of positive tunel - staining cells and inhibited caspase - 3 activation
通道阻断剂100nmibtx或100nmchtx均有显著的神经元保护作用，可减少tunel阳性细胞比例及抑制caspase上激活；然而， a型钾通道阻断剂4apo0qm ）或skc 。 |
