| 1. | Cloning and sequence of - galactosidase gene of lactobacillus burgarlus
半乳糖苷酶基因克隆和序列分析 |
| 2. | Cloning and expression of - d - galactosidase from coffee bean coffea liberica amp; amp; coffea canephora
半乳糖苷酶基因的克隆与表达 |
| 3. | The effect of - galactosidase and polygalacturonase on peach ripening and softening
半乳糖苷酶及多聚半乳糖醛酸酶对桃果实成熟软化的影响 |
| 4. | We succeeded in constructing the fusion protein plasmids of ea and ed of p - galactosidase . 2
构建的质粒能高效表达具有较高a一互补活性的gs …蛋白、 gsthd蛋白。 |
| 5. | The a - complementation reaction of & - galactosidase ea and ed was also used in dna cloning , protein protein interactions monitoring and expressing immunoassays studying
-半乳糖苷酶的ea 、 ed这种-互补性还被用于分子生物学、蛋白质相互作用的监控、表达免疫分析等方面的研究。 |
| 6. | Then the linked products were transformed into the high competent cell of e . coli dh5a . based on - complementation of the detective - galactosidase , positive recombinant clone were screened from x - gal plate
从引物和基因序列的比对分析结果看， zhyf006序列与上下游引物的配对比例分别为s4 |
| 7. | Ability to a - complement of the ea / ed protein was determined by the addition of onpg western blot test with rabbit to e . coli p - galactosidase pcab as first antibody was used to verify the fusion proteins
以兔抗p半乳糖昔酶抗体做western blot以证实与gst融合表达的ea工d蛋白是p半乳糖昔酶的成分。实验结果1 |
| 8. | P - galactosidase of e . coli which is scarce in human blood plasma and has a variety of substrates , is one of the most frequently used enzyme labels . it is used both in heterogeneous and homogeneous enzyme immunoassay
大肠杆菌的-半乳糖苷酶，因人血浆中缺乏此酶，并具有大量易得的底物，被用于均相及非均相免疫分析，是目前最常用的标记酶之一。 |
| 9. | Recombinant fpvs were selected and purified by blue plaques expressing 3 - galactosidase . . the expression of foreign genes in rfpvs were confirmed by ifa . trails for evaluating protective efficacy of rfpvs against very virulent mdv or aiv challenge
纯化后脂质体转染，蓝斑筛选纯化得到稳定的重组病毒rfpv - ps - ha - pe / l - f ，间接免疫荧光法证实， ha基因和f基因同时得到了表达。 |
| 10. | Cloning and expression in e . coli of lactase . specific primers were designed according to the sequence of the beta - galactosidase gene from kluyveromyces lactis . klac gene was amplified by pcr and subsequently cloned
乳糖酶基因的克隆及原核表达以乳酸克鲁维斯酵母( kluyveromyceslactis )菌株k538的基因组dna为模板，设计引物，利用pcr获得乳糖酶基因klac ，经dna测序验证，得到克隆t1549 。 |
