| 1. | Some virulent strains of pvx cause rugosity go the leaves even crinkling, called pvx crinkle .
PVX的有些强毒株系引起叶子的粗缩甚至皱缩，叫做PVX皱缩。 |
| 2. | Some virulent strains of pvx cause rugosity go the leaves even crinkling , called pvx crinkle
Pvx的有些强毒株系引起叶子的粗缩甚至皱缩，叫做pvx皱缩。 |
| 3. | There were six major open reading frames in genome of f48e9 strain which encode the viral np , p , m , f , hn and l protein , respectively
为了验证该6个碱基是否只存在于强毒株及其与毒力的关系，我们又设计一对引物x1和x2 。 |
| 4. | Indirect immuno - fluorescent assay and enzyme linked immunosorbent assay ( elisa ) showed that the expressed protein was reactive with chicken anti - ibdv serum
Dna疫苗对bc6 85 、 zj2000 、 zj991等3株强毒株具有良好的免疫保护效果，平均保护率达83 |
| 5. | The ha 1 gene was inserted into the bacterial plasmid pgex - 4t - 2 and the recombinant plasmids containing ha 1 gene were identified by restriction enzyme analysis and pcr mathod
结果表明同源性分别达到98和97 ，并且在ha1切割位点有多个碱性氨基酸的插入序列，证明其为强毒株。 |
| 6. | The results showed that samples confined non - virulent apmv - 1 can yield two bands of 123bp and 362bp , while those of virulent ndv can yield two bands of 254bp and 362bp
对强毒株可扩增出362bp的通用片段和254bp的强毒株特异性片段，弱毒株则可扩增出362bp的通用片段和123bp的弱毒株特异性片段。 |
| 7. | There is minor variation in some nucleotide sequences . and the results indicate by analysis that the deduced amino acid sequence contains characteristic sequence of splitting site of high pathogenic ai strain
同时分析表明该核苷酸含有强毒株裂解位点的特征性序列，从而从分子水平证明了该病毒分离株为高致病力毒株。 |
| 8. | Haake da , champion ci , martinich c , et al . molecular cloning and sequence analysis of the gene encoding ompl1 , a transmembrane outer membrane protein of pathogenic leptospira spp [ j ] . j bacteriol , 1993 , 175 ( 13 ) : 4225
晏菊芳,鲍朗,伍卫华,等.中国钩端螺旋体强毒株017膜蛋白基因的质粒载体构建及表达[ j ] .中华微生物学和免疫学杂志, 1999 , 99 ( 2 ) : 117 |
| 9. | The traditional inactivated or attenuated vaccines could not provide enough protection against these variants and wibdv . therefore , a new generation of more effective and safer vaccines is anticipated . the purpose of this study was to develop a dna vaccine against ibdv
用弱毒苗和灭活苗免疫鸡群是目前防治ibd的主要方法，但随着ibdv变异株与超强毒株的出现，免疫失败常有发生。 |
| 10. | Two amino acid substitutions near the vp2 - vp4 cleavage site were identified in the virulent strain jz2000 and might be involved in increased virulence of the virus . phylogenetic analyses indicated that these two strains were most closely related to some european strains but distinct from wibdv and variant strains
分子系统进化树分析表明， zj2000株和jd1株与cu - 1 、 p2 、 cef94 、 harbin等毒株的关系最近，而与欧洲、香港、日本的超强毒株和美国的变异株相对较远。 |
