| 1. | The tandem expression of the human salmon chimeric ct gene in e . coli
鲑降钙素嵌合基因在大肠杆菌中的串联表达 |
| 2. | Construction and identification of dna vaccine containing chimeric gene gag - gp120 of hiv
120嵌合基因核酸疫苗的构建与鉴定 |
| 3. | Construction of an expressing vector for antisense rna - ribozyme chimeric dna sequence against tomato acc synthase gene and transformation of tomato
核酶嵌合基因植物表达载体的构建及对番茄的转化 |
| 4. | Therefore , toreniafournieri is a very suitable model plant for studies of double fertilization in living material . we transferred the fusion gene ofgfp : mtn to toreniafournieri by leaf disc transformation mediated by agrobacterium and gain transgenic plant
我们将嵌合基因gfp : mtn通过叶盘法导入蓝猪耳，探索了蓝猪耳遗传转化的方法和转基因苗的再生条件，建立了成熟的蓝猪耳转化系统。 |
| 5. | Owing to the common expression of egf in various organs of mouse , it could be deduced that the role of egf was mainly autocrine and paracrine actions , and its endocrine role was also not excluded owing to its high expression abundance in submaxillary gland
由于egf在小鼠各器官中表达的普遍性，可以推测egf的作用以旁分泌和自分泌作用为主，但它在颌下博士学位论文“ tnegfalelittin ”嵌合基因构建及有关基因重组表达的研究等器官中的高表达表明其也具有内分泌作用。 |
| 6. | Based on the structure and function analysis of hirudin , a potent thrombin inhibitor , and some platelet aggregation inhibitors , which contain the recognition sequence argglyasp as their functional motif , two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus . these chimera genes were constructed by pcr and inserted into the expression vector pet21a , the constructs were confirmed by restriction enzyme digestion and dna sequence analysis . these recombinant plasmids were transformed into
经限制酶消化和dna序列分析，证明两种重组质粒与设计完全一致。由于rgd -水蛭素嵌合基因上游连接了金黄色葡萄球菌蛋白a spa的信号肽序列，在iptg诱导下两种嵌合分子都获得了分泌表达，表达产物主要集中在细胞周质空间。 |
| 7. | Furthermore , by inserting " anther box " element to the mutated area of two site - mutation promoters , another two promoters , ipmas and ipmal , were created . in order to study the chemical - inducible capacity of wild and modified pr - la promoters , a coding sequence of gus ( | 3 - glucuronidase ) gene was fused to their downstre am , and the chimeric genes were cloned into pbin ! 9 - based plant expression vector
为了检测得到的启动子驱动效率及诱导活性，将所得到的启动子、定点突变启动子和插入花药盒的启动子与gus基因连接，构建了6个植物表达载体，同时分别构建包含ipl barnase 、 ipml barnase 、 ipmal barnase嵌合基因的植物表达载体。 |
| 8. | 2 . an anther specific chimaeric male sterile gene expression box with a enhanced promoter ( ta29 ) driving coda gene was constructed and the expression box was inserted into binary vector p3301 that contains a l - phosphinothricin ( ppt ) - resistant selective marker gene and - glucuronidase ( gus ) reporter gene in t - dna region
以增强的ta29启动子驱动克隆的coda基因，构建成花药特异性嵌合基因表达盒；将此表达盒插入双元载体p3301 ，构建成以ppt抗性基因为选择标记，以gus为报告基因的植物表达载体。 |
| 9. | The characteristics of tm - 22 expression presented in transgenic tobacco : 1 ) . virus specificity in either homozygote or heterozygote ; 2 ) tm - 22 gene integrated in tobacco genomic dna with single copy and in inheritance and segregation to progenies on the mendel role ; 3 ) . transgenic line with tm - 22 promoter ( ptm47 ) showed infected symptoms with cell death distinguished to one with 35s promoter ( ptm49 ) after inoculation with tomv - 2a
其次，通过氨基酸序列和结构的比较，确定tm - 2 ~ 2基因的编码蛋白与tomv病毒在抗病反应中相互识别的特异氨基酸及其功能；然后，应用重组dna技术，互换tm - 2 ~ 2基因和tm - 2基因的对应结构域，构建嵌合基因，获得嵌合蛋白表达的转化体，验证tm - 2 ~ 2编码蛋白中变异氨基酸的作用。 |
