| 1. | In contrast , most of the pollen grains in the non - transformant have plump shape and were stained into red color uniformly
转正向表达载体在烟草正常花粉粒的百分比较对照植株降低了42 。 |
| 2. | Southern blot analysis suggest that s2 gene has been integrated in the genome of three pcr - positive regenerated plants . the rest two regenerated plants did not show a band corresponding to s2
取这3株的嫩叶，对照植株( 2株)的嫩叶，作hplc分析， 3株转基因植株中均检测到白藜芦醇的存在。 |
| 3. | Under high light treatment , the f ormation of a and z decreased , and the values of non - photochemical quenching ( npq ) and fv / fm in a29 were both lower than those in the wild - type plant
通过冻融法从该植株的类囊体中提取vde酶，其酶活性为3 2 ，是对照植株的45 7 ，表明vde酶受到了抑制。 |
| 4. | After 60 days of growth the leaf net weight of transgenic tobacco plants has increased by108 . 4 % . 112 . 7 % respectively compared to wild - type controls . furthermore , chlorophyll in transgenic plants has risen by 34 % . 38 % compared to wild - type controls
5与对照相比，转基因烟草表现出叶片生长和叶绿素含量增加：在生长了60天之后，转基因烟草植株比对照植株相同部位叶片的平均干重分别增加108 . 4 |
| 5. | Putative transgenic plants were screened b y nptii - specific and mnsod - specific pcr amplification and southern blotting , 84 % of the transgenic plants gave positive results . the results of mnsod activities demonstrated that tobacco mnsod gene contributed to more than 50 % of the total mnsod activity in some transgenic alfalfa
Npt基因和mnsod基因的pcr检测和southern杂交表明mnsod基因已经整合到84的保定苜蓿转基因植株的基因组中， mnsod活性测定结果表明转基因植株中的mnsod活性与对照植株之间存在显著差异，部分转基因植株的mnsod活性比对照植株提高了1倍以上。 |
| 6. | Five plants ( tx0014 ) regenetated from selection medium were obtained . the . presence of the stilbenesynthase gene 2 in the regenerated plants was checked by pcr . all of the plants showed a clear band corresponding to the relevant sequence of the gene , but the control showed no band
通过kan生根筛选，获得转芪合酶基因( s2 )的番茄tx0014品种5株，提取这5株转基因植株和一株对照植株的总dna ，作pcr检测， 5株均呈阳性，而对照植株无特异片段出现，继续southern检测，结果表明，芪合酶基因( s2 )已整合到其中3株的基因组中。 |
| 7. | To make the ti plasmid pbi121 - nhap for transformants the tobacco ( nc89 ) , at the same time to make the pemu - mcs - n - nhap and pwubi . tml - nhap for transformants the yan wheat 18 . we getted 4 transgenic tobaccos , measured the ability of salt tolerance by treatment in 150 mmol / 1 nacl , found that the transgenic tobaccoes can growth and the control tobaccos ca n ' t
通过比较转synnhap基因烟草与对照烟草的耐盐性，发现4株转synnhap基因的烟草再生苗在含150mmnacl的培养液中处理两周后，植株外观无明显变化，叶片生长情况正常，而对照植株的生长则受到抑制，叶片明显萎蔫、失绿并最终脱落。 |
| 8. | Moreover , local lesion assay on chenopodium amaranticole showed that the virus titer in the transgenic tomato plants were significantly lower than that of the non - transgenic plants . the collective results demonstrate that ttom1 is a host factor involved in the replication and / or maintenance of cmv in tomato
结果表明，转反义ttom1番茄植株产生的枯斑数比非转基因番茄植株的枯斑数显著减少，表明转基因番茄中cmv的浓度要明显低于非转基因对照植株，证明番茄ttom1基因是与cmv的复制效率有关的一个寄主因子。 |
