| 1. | Site - specific mutagenesis of murine anti - human tnf - fab gene and its expression in e . coli
基因的定点突变及其在大肠杆菌中的表达 |
| 2. | In the work , we got mutated plasmids pgex - 6p - m - centrein by means of pcr and recombinant dna techniques
随着生物技术的逐步成熟，定点突变成为了科研人员进行科学研究的有力手段。 |
| 3. | Purple clones were picked out from the plate , which show br was expressed . pcr analysis told us that the mutant br gene was transformed into l33
Br基因的定点突变改造和突变基因在嗜盐菌中表达系统的建立使我们可以研究br的各种突变蛋白。 |
| 4. | The - 1 , 4 - gt gene was got from the plasmid pmgt - 239 / 2615 through pcr techniques , and cloned into the pgemt vector . its sequence was determined by dna sequencing system
通过- 1 ， 4 - gt的定点突变改变其功能，从而以此为靶标进行药物筛选。 |
| 5. | Protein engineering and site directed mutagenesis have been used to change the active site and alter the substrate specificity of various hydroxynitrile lyases
研究人员已经利用蛋白质工程和定点突变技术来改变各种醇腈酶的活性位点和底物特异性等。 |
| 6. | The principles , operation and applications of site - directed mutagenesis , gene fusion technology , and post - translational modification methods were introduced emphatically
着重阐述了基因定点突变技术、基因融合技术和翻译修饰技术等新兴定点固定化技术的原理、特点和操作。 |
| 7. | According to three - dimensional structure modeling , we select two mutants : p163a and d92l . the mutant recombinant plasmid pplc3 . 5k - pel - p163a was expressed in pichia pastoris gs115 and smd1168
二、 pel基因的定点突变定点突变获得突变基因pel - p163a 、 pel - d92l ，并在毕赤酵母中表达。 |
| 8. | By artificially changing a to c at - 137 bp site upstream from transcription start point of cloned promoter , two site - mutation promoters , ipms ( 603 bp ) and ipm1 ( 900 bp ) were created
通过pcr方法对克隆的两个启动子进行定点突变，使转录起始位点上游- 137bp处a突变为c ，得到两个突变启动子( ipml 、 ipms ) 。 |
| 9. | Phylogenetic tree indicates the rab protein may play a role in vesicular trafficking from endoplasmic reticulum to golgi body . two tga in eo - rabl were successfully mutated to tgc by site direct pcr procedure and the truncate eo - rabl was obtained
以此重组质粒为模板进行pcr定点突变，将eo - rab1基因中前两个tga突变为通用半胱氨酸密码子tgc ，获得截短型eo - rab1基因。 |
| 10. | Conclusion we have constructed the expression plasmid pbv220 - hpf4 successfully . 3 ' - utr of h pf4 c dna was deleted and tag was mutated to taataa by pcr . after sds - page and densitometric scan analysis , the expression level of r hpf4 is 25 - 30 %
结论本研究运用pcr定点突变技术，完全去除了hpf4cdna基因3 ”端utrat富含区：改用大肠杆菌强串联终止密码子taaaataa ，成功构建高效表达克隆pbv220 rhpp4 。 |
