Research advance of transgenic techniques of transducing foreing dna through sperm 精子作为外源基因载体的转基因方法研究进展
2.
Objective to review the development of genic therapy with the nanoparticle as carrier 摘要目的综述以纳米颗粒作为基因载体进行基因治疗的发展概况。
3.
Cut pgah with hindlll into linear plasmid as vector fragment and insert beta fragment into it . the recombinant vector with dualt salt - tolerance gene beta and coda was constructed 用hind单酶切pgah质粒作为载体片段，将beta基因片段作为插入片段与载体片段相连，即构建出双耐盐基因载体。
4.
The recombinant plasmid pbmb1205ac was obtained . recombinant plasmid of puc19g was constructed when pcr product of gfp was digested by sphl and inserted into puc19 . the gfp fragment with sphl and bglii enzyme sites from puc19g was inserted into pbmb1205ac 同时扩增全长gfp片段，将扩增的gfp片段用sph消化插入到puc19上得到puc19g ，再利用sph 、 bgl双酶切消化puc19g ，回收两端酶切位点分别为sph 、 bgl的gfp片段，将得到的gfp片段插入到pbmb1205ac上最终得到重组的融合基因载体pbmb0474 。
