| 1. | To establish a biochemical method to find dnaase in earthworm ; 2
建立双胸蚓dna酶分离纯化的技术体系； 2 |
| 2. | The crude sample which had been diluted 54 times could decompose 1 ul dna ( 0 . 596 ug / ul ) completely at 37 @ in a hour
54倍稀释的双胸蚓粗提取液37c保温1小时可将ivl人dna门569ug vl ）完全消化。 |
| 3. | 3 . to study physical and chemical property of dnaase the purified dnaase reacted with circular pbv220 - y - inf for an hour at 37 , then the producetion was detected by 1 % agrose gel
双胸蚓组织中dna酶的酶学性质研究将纯化的dna酶分别与环状pbv220 - ? inf37反应1小时后，用1的琼脂糖凝胶电泳对其反应产物进行观察。 |
| 4. | The finding of dnaase in the earthworm the tissue extract of earthworm which had been diluted 28 times could digest 4 ul pbv220 - r - inf ( 6 . 66 u g / ul ) completely at 37 @ in a hour but it had no effect on rna
双胸蚓组织中dna酶的发现28倍稀释双胸蚓组织粗提液37保温1小时，可将4vlpbv220 。 y一inf质粒（ 6石6119 11l ）完全消化但对rna无消化作用。 |
| 5. | Under the same condition , the tissue extract of earthworm and the tissue protein extract of earthworm could decompose the pbv220 - r - inf completely but the tissue extract of earthworm without protein could n ' t . 2
双胸蚓组织粗提取液及双胸蚓组织的蛋白提取液在37oc保温1小时可以完全消化pbv220 p一inf质粒而双胸蚓组织去蛋白提取液对pbv220 y一wf质粒无消化作用。 |
| 6. | The isolation and purification of dnaase in the earthworm the earthworm dnaase was purified from the tissue extract of earthworm by denaturing the protein with low ph buffer , high temperature , ammonium sulfate precipitation , deae - cellulose ( de52 ) chromatography and ultra - filter membrane
双胸蚓组织中dna酶的分离纯化双胸蚓组织粗提取液经过选择性酸变性、选择性热变性、硫酸按分段盐析、 deae一纤维素（ de52 ）柱层析、超滤膜分级分离后得到一个电泳纯的dna酶。 |
| 7. | To isolate and purify dnaase in the earthworm first , the tissue extract of earthworm was prepared by dissolving the earthworm with sucrose and denaturing the protein with low ph buffer . then dnaase was purified by denaturing the protein with higher temperature . the following steps were ammonium sulfate precipitation , deae - cellulose ( de52 ) chromatography and filtration by ultra - filter membrane
双胸蚓组织中dna酶的分离纯化采用蔗糖溶解双胸蚓，并选择性酸变性制备双胸蚓组织粗提取液，再经选择性热变性、硫酸铵分段盐析、 deae ?纤维素( de52 )柱层析及超滤膜分级分离对双胸蚓组织中dna酶进行分离纯化。 |
| 8. | To find dnaase in the earthworm the tissue extract of earthworm with different concentration reacted with rna , circular dna , linear dna for an hour at 37 , then the producetion was detected by 1 % agrose gel . the tissue extract of earthworm , the tissue protein extract of earthworm and the tissue extract of earthworm without protein reacted with pbv220 - r - inf for an hour at 37 , then the producetion was detected by 1 % agrose gel . 2
双胸蚓组织中dna酶的发现用不同浓度的双胸蚓组织提取液与rna 、环状dna及线状dna在37反应1小时后用1的琼脂糖凝胶电泳对其反应产物进行观察；双胸蚓组织粗提取液、双胸蚓组织蛋白粗提取液及双胸蚓组织去蛋白提取液分别与pbv220 - ? inf质粒37反应1小时后用1的琼脂糖凝胶电泳对其反应产物进行观察。 |
