| 1. | Highly conserved sequence 高度保守序列 |
| 2. | The results indicate that conserved motif glpl and cfly does not exist in ht gene , in other words , ht gene does not belong to the type of nbs - lrr resistance gene 说明玉米ht基因不含绝大多数r基因特有的两个保守序列glpl和cfly ,或者说ht基因不属于nbs - lrr类r基因。 |
| 3. | The nucleotide acids in both conserved sequences are mutative , even the length of the spacer is variable , and they both brought troubles in determining escherichia coli promoter with computer 由于保守序列中的碱基是可变的,而且间隔碱基的长度也是可变,这给大肠杆菌启动子的计算机识别带来了难度。 |
| 4. | A pair of primer , dig labeled probe and a taqman probe based on the conserved nucleotide sequence of cp gene of different pnrsv strains were designed and synthesized 本文根据病毒各株系外壳蛋白基因的保守序列,设计了杂交诱捕探针、 dig标记杂交探针以及确定了最佳诱捕参数和杂交检测参数,建立杂交诱捕rt - pcr ? elisa 。 |
| 5. | At the n - terminal end is the variable domain of 26 amino acids in length , which has no conserved mgxxc ( s / q ) xxt sequence essential for n - myristoylation . so it is conferred that the vfcpkl is a soluble protein N端的可变区有26个氨基酸残基,没有豆蔻酰化所必需的保守序列mgxxc ( s q ) xxt ,因此推测vfcpk1可能是水溶性蛋白。 |
| 6. | With a pair of degenerate primers designed according to the conserved region among the known cdna sequences of five rodents , a cdna fragment was generated by rt - pcr from total rna isolated from ovaries of lagurus lagurus 首先根据已发表的五种啮齿目动物zp3cdna序列同源性分析结果设计特异性引物, rt - pcr扩增草原兔尾鼠zp3 ( lzp3 ) cdna保守序列。 |
| 7. | Signal protein of sh2 domain ; sh2 domain is a conservative sequence containing of 100 amino acids . similar sequences were found in many proteins . they become a family of proteins with sh2 domain 结构域信号蛋白sh homoloy2 )结构域是由100个氨基酸组成的保守序列,在许多细胞内信号蛋白中发现了相似序列,它们因与src基因表达产物src蛋白有相应的结构域而得名。 |
| 8. | Based on the 5 " end conservative sequence of grb - ast precursor cdna and the adaptor sequence , two gene specific primers ( gsp ) and an adaptor primer ( ap ) were synthesized respectively for the cloning of signal peptide 首先,根据已报道的grb - ast前体cdna靠近5端的保守序列,设计合成2条基因特异引物,并根据接头序列设计合成一条接头引物,用于信号肽序列的克隆。 |
| 9. | Two degenerate primers were designed and synthesized according to the highly conservative sequences among the known - glc genes . a cdna fragment of 208bp was amplified by rt - pcr , which was subsequently cloned into pmd18 - t vector for sequencing analysis 利用genbank中已经登录的其它植物中该酶基因的保守序列,设计一对简并引物,采用rt - pcr技术,从茶树扩增出208bp的cdna片段。 |
| 10. | According to the sequences of the conservative region of avain chlamydia momp genes reported by people abroad , one pair of primers were designed to amplify momp gene with pcr whose template was genome dna of this strain , then it was sequenced 其次,根据国外报道的禽衣原体momp基因两端保守序列设计合成一对引物,以提取的衣原体基因组dna为模板, pcr扩增、克隆了momp基因,并对其进行了测序。 |