| 1. | Among all pgc - derived cultures , it was found that one derivation under went 12 passages
具有分化潜能的pgcs能在体外连续传代培养达12代。 |
| 2. | The culture of hela cervical carcinoma cell line and bcap - 37 breast carcinoma cell line
宫颈癌hela细胞系和乳腺癌bcap上7细胞系传代培养； ihp wgz第四军医大学硕士学位论文3 |
| 3. | Effects of cell density on proliferation and osteogenic differentiation of human mesenchymal stem cells during subculturing in vitro
传代培养密度对人间充质干细胞增殖及成骨分化的影响 |
| 4. | After repeated adherence and enzyme digestion , the cells attained the requirement of morphological purification with a purity of more than 95 %
经台盼蓝拒染计数，将细胞按1 10 ^ 6接种于培养瓶，传代培养。 |
| 5. | The smg cell of rats were isolated and purified by pancreation digestin and then were cultured and subculfured in dmem with 20 % fetal bovine serum
应用胰酶消化法进行颌下腺细胞的分离、纯化及原代培养、传代培养。 |
| 6. | Continuous passage of neurospheres was used to isolate , culture nscs from dentate gyrus of hippocampus of newborn rats and to observe the self - renewal of neurospheres , which were immunostained with nestin
采用神经球连续形成法从新生大鼠海马齿状回分离、培养神经干细胞，通过连续传代培养观察其自我更新能力。 |
| 7. | With the increase of passage , the total of colonies decreased , but the number of classical nest - like or island - like eg cell colonies relatively increased . the growth of eg cells was dependent on the feeder layer
传代培养的eg细胞对饲养层有依赖性， icr品系小鼠pgc细胞和由其传代后获得的eg细胞呈明显的群聚性，以单个存在状态的较少 |
| 8. | There was about 7 days interval between every two passages . eg cell clones can be found in each primary culture from those embryos of appropriate age . in culture of one embryonic cell line , eg cell clones maintained after 9 passages
在实验中，几个适龄的胚胎的原代培养物中都出现了鸟巢状的eg细胞集落，维持时间最长的一个胚胎细胞株在传代培养了9代之后仍然可以观察到eg细胞集落。 |
| 9. | In 96 - well plates , effects of nutritional factors including glutamine , pyruvic acid , iron citrate , silicon , rpmi 1640 and marine broth 2216 were examined on the viable cell density in primary cell culture of s . domuncula after 36 hours of inoculation
长期培养发现了adcp一p ~ orph中的骨针生长等现象，进一步证明了adcp一pri ~ orph中旺盛的代谢活动。通过两种方式进行adcp一pri ~ orph传代培养的初步探索。 |
| 10. | 3 after incubated with 5 - bromo - 2 - deoxyuridine ( brdu ) for 7 days , newborn cells derived from regenerative neurospheres were immunostained . 4 the cells were collected by centrifugation separation and put into poly - 1 - lysine - coated terasaki wells , which were randomly divided into one of three groups of 24 wells cells each
4将传代培养的神经球离心收集，转移至24孔培养板，随机分为3组，每组24孔细胞，置5 % coz 、 370c培养箱，连续培养7d ，观察神经球的诱导分化。 |
